Annual limits imposed last week have effectively closed grouper-fishing areas that are 300 feet or deeper until January.
The new rules halted deep water grouper harvesting, which reached the one million-pound quota.
The quota for shallow-water grouper is 8.8 million pounds, only 5.31 million pounds of which can be red grouper.
Quotas also affect shallow-water recreational fishermen who are now limited to two red grouper per trip, instead of five.
The quotas are a first step in a ten-step plan to stop overfishing in the Gulf of Mexico; though regulators and fishermen agree it is difficult to predict the quotas' effects.